Table 1 The sequences of primers used for quantitative real-time polymerase chain reaction
Gene name | Primer sequences |
|---|---|
miR-195-5p | F: GGGGTAGCAGCACAGAAAT |
R: CTGGTGTCGTGGAGTCGG | |
U6 (internal reference of miRNA) | F: CTCGCTTCGGCAGCACA |
R: AACGCTTCACGAATTTGCGT | |
Runt | F: CGGGCAATGACGAAAAC |
R: GCTCGGAAAAGGACAAACT | |
RUNX2 | F: GGACGAGGCAAGAGTTTCAC |
R: ACTGGGATGAGGAATGCG | |
bone morphogenetic protein (BMP) 2 | F: CGAGAAAAGCGTCAAGCC |
R: CCACATCACTGAAGTCCACATAC | |
alkaline phosphatase (ALP) | F: GGTGTCGGAAGATGGGA |
R: CAAAGGAATGTTAGGGGC | |
osteocalcin (OCN) | F: GGAGGGCAGTAAGGTGGTGAA |
R: GAAGCCAATGTGGTCCGCTA | |
Smad7 | F: GTGCGTGGTGGCATACTGG |
R: CGATCTTGCTCCTCACTTTCTG | |
GAPDH (internal reference of mRNA) | F: CCACTCCTCCACCTTTG |
R: CACCACCCTGTTGCTGT |