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Fig. 1 | BMC Cardiovascular Disorders

Fig. 1

From: Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Fig. 1

miR-195-5p promotes the osteogenic differentiation of vascular smooth muscle cells (VSMCs) via the Wnt/β-catenin pathway. (A) Cell viability was determined using an MTT assay. (B, O) Cell apoptosis was determined using flow cytometry. (C–I) The levels of Runt, RUNX2, BMP2, ALP, OCN, and Smad7 mRNAs and miR-195-5p were determined using RT-qPCR. (J–N) The levels of RUNX2, Smad7, Wnt3a, and β-catenin proteins were determined using western blotting. (P) Alkaline phosphate staining. (Q) Alizarin Red staining (×200). Scale bar, 1000 μm, *p < 0.05 vs. 0 h group, #p < 0.05 vs. 3 h group, and p < 0.05 vs. 7 h group

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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