Fig. 8

Silencing HAPLN1 activated the PKA signaling pathway in HF rat model (n = 6). (A) HAPLN1 knockdown reduced MDA level and elevated GSH level in the HF rat model. (B) HAPLN1 knockdown inhibited the expression of apoptosis-related factors, including LDH, TNF-α, and IL-6 in the HF rat model. (C) In the HF rat model, silencing HAPLN1 reduced CAMKII protein phosphorylation and promoted PLB protein phosphorylation. The results are presented as the mean ± SD from three independent experiments. ^**P < 0.01, ^***P < 0.001 vs. sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. HF + sh-NC group