Fig. 6

Silencing of HAPLN1 activated the PKA signaling pathway in DOX-induced AC16 cells (n = 3). (A) The Venn diagram illustrated the 351 genes common to HAPLN1 co-expressed genes and those associated with HF. (B) Bubble plot of KEGG enrichment analysis of 351 shared genes. (C) Western blot revealed that the protein expressions of PKA pathway-associated protein PKA was increased, while PKC and cAMP were decreased after HAPLN1 knockdown in DOX-induced AC16 cells. (D) HAPLN1 silencing reduced protein phosphorylation of CAMKII and promoted protein phosphorylation of PLB in DOX-induced AC16 cells. The results are presented as the mean ± SD from three independent experiments. ^***P < 0.001 vs. Control group; ^#P < 0.05, ^###P < 0.001 vs. sh-NC + DOX group. Notes DOX, doxorubicin