Fig. 4
The expression of PCNA and MMP-9 in VSMCs. 1: Control group, 2: AngII (10− 6mol/L) group, 3: AngII (10− 6mol/L) + Entresto (10− 5mol/L) group. (3A) Entresto was used for 1 h before Ang II stimulation for 24 h, and then the expression of PCNA and MMP-9 at mRNA level was detected by qRT-PCR. (3B) Entresto was used for 1 h before Ang II stimulation for 24 h, and then the expression of PCNA and MMP-9 at the protein level was observed by Western Blot. (3C) This is the quantization diagram. Mean ± SEM (n = 3). a: p < 0.05, compared with the control group. b: p < 0.05, compared with Ang II group