Fig. 4

IL1β production-related post-infarction cardiac inflammation was suppressed by CoQ10 treatment. (A) Analysis of gene expression in the infarct myocardium from each group of mice at 3 days after sham/LAD-ligation surgeries (Statistical analysis was performed using 2-way ANOVA with Sidak’s multiple comparison tests). Mice were treated with either vehicle or CoQ10. (B)(C) Representative images and analysis of the IL1β immunofluorescence staining in the Mac3-positive macrophages from mice treated with vehicle or CoQ10 at 28 days after LAD ligation (Statistical analysis was performed using a two-tailed unpaired Student’s t-test). 630×. Scale bars indicate 20 μm. (D) Representative flow cytometry analysis of left ventricles after sham/LAD ligation surgeries. Cells were defined as neutrophils (Neut, CD45.2⁺CD64⁻Ly6G⁺), monocytes (Mono, CD45.2⁺Ly6G⁻CD64^intLy6C^hi), macrophages (Mac, CD45.2⁺Ly6G⁻CD64⁺Ly6C^lo), or CCR2⁺ macrophages (CCR2⁺Mac, CD45.2⁺Ly6G⁻CD64⁺Ly6C^loCCR2⁺). (E) Quantification of the mean fluorescence intensity (MFI) of IL-1β in the CCR2⁺ macrophages of mice 3 days after LAD ligation, either treated with vehicle or CoQ10 (Statistical analysis was performed using a two-tailed unpaired Student’s t-test). MI: myocardial infarction; CoQ10: Coenzyme Q10; MFI: mean fluorescent intensity. *p < 0.05, **p < 0.01, ***p < 0.001