Fig. 2

Mice with CoQ10 treatment have a better survival rate and attenuated cardiac remodeling after LAD ligation. (A) Kaplan-Meier survival curve of mice administrated with Vehicle or CoQ10 following LAD ligation (Statistical analysis was performed using the log-rank test). (B)(C) Representative echocardiographic images and analyses of mice at 28 days after sham or LAD ligation surgeries. Mice were administered with either vehicle or CoQ10 (Statistical analyses were performed using 2-way ANOVA with Tukey’s multiple comparison tests). (D)(E) Representative images and analysis of WGA staining of the heart sections from mice of each group at 28 days after sham or LAD ligation surgeries, administrated with vehicle or CoQ10. 200×, scale bars indicate 100 μm (Statistical analysis was performed using 2-way ANOVA with Tukey’s multiple comparison tests). (F)(G) Representative images and analysis of Masson’s trichrome staining of the heart tissue sections from mice of each group at 28 days after sham or LAD ligation surgeries, administrated with vehicle or CoQ10. Scale bars indicate 1 mm (Statistical analysis was performed using 2-way ANOVA with Sidak’s multiple comparison tests). (H) Serum BNP levels of mice at 28 days after sham or LAD ligation surgeries. Mice were administered with either vehicle or CoQ10 (Statistical analysis was performed using 2-way ANOVA with Sidak’s multiple comparison tests). (I) Quantification of flow cytometry analysis of myeloid immune cells in the left ventricles at 28 days after sham/LAD ligation surgeries (Statistical analysis was performed using 2-way ANOVA with Tukey’s multiple comparison tests). Mice were treated with vehicle or CoQ10. MI: myocardial infarction; CoQ10: Coenzyme Q10; EF, ejection fraction; LV: left ventricle; CSA: cross-sectional area of myocyte; BNP, brain natriuretic peptide. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001