iPSCs applications | Methods | Value | References |
---|---|---|---|
Genome editing | Gene correction | Generation of isogenic controls for disease modelling | |
Gene insertion | Generation of novel mutation | ||
Gene knockdown | Perform eQTL studies | [67] | |
CRISPR-mediated gene interference (CRISPRi) or gene activation (CRISPRa) | Investigate GWAS risk variants function | ||
CRISPR-based screening | Investigate known genetic modifiers | ||
Identify novel therapeutic targets and biomarkers | |||
3D cell models | Organoids | Mimic organ functions in vitro | |
Replicate the pathology in vitro and discover underlying pathologic mechanisms | |||
Develop high-throughput screening platform | [80] | ||
Engineered tissues i.e., Vascular rings | Mimic organ functions in vitro | ||
Replicate the pathology in vitro | [84] | ||
Organ-On-a-Chip | Mimic organ functions | ||
Model the pathology and discover therapeutic targets/pathways | |||
Develop platform for high-throughput screening | |||
Multi-omics | Genomics, Epigenomics, Transcriptomics, Proteomics, Metabolomics | Provide insights into disease mechanisms | |
Identify potential diagnostic biomarkers | [94] | ||
Drug screening | Phenotypic screening of drug libraries | Uncover underlying therapeutic targets/pathways | [80] |
Test effectiveness of candidate drugs | |||
Novel compounds | Test drug toxicity | [96] | |
Test drug permeability |